Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase.
نویسندگان
چکیده
The Escherichia coli aroE gene encoding shikimate dehydrogenase was sequenced. The deduced amino acid sequence was confirmed by N-terminal amino acid sequencing and amino acid analysis of the overproduced protein. The complete polypeptide chain has 272 amino acid residues and has a calculated Mr of 29,380. E. coli shikimate dehydrogenase is homologous to the shikimate dehydrogenase domain of the fungal arom multifunctional enzymes and to the catabolic quinate dehydrogenase of Neurospora crassa.
منابع مشابه
Cloning of cDNA encoding the bifunctional dehydroquinase.shikimate dehydrogenase of aromatic-amino-acid biosynthesis in Nicotiana tabacum.
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 249 2 شماره
صفحات -
تاریخ انتشار 1988